Review



mix n stain cf568 antibody labeling kit  (Biotium)


Bioz Verified Symbol Biotium is a verified supplier
Bioz Manufacturer Symbol Biotium manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    Biotium mix n stain cf568 antibody labeling kit
    Mix N Stain Cf568 Antibody Labeling Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cf568+mix+n+stain+kit/Mix-n-Stain+Biotin+Antibody+Labeling+Kit/bio_rxiv__2025__09__12__675813-48-11-16
    Average 95 stars, based on 95 article reviews
    mix n stain cf568 antibody labeling kit - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Concentration Assay:

    Article Title: Protocol for cytoskeleton staining of the semi-adherent multiple myeloma cell line RPMI 8226 by immunofluorescence.
    Article Snippet: Approach for minimal distortion of cell morphology by reducing centrifugation Preservation of fine cellular details of semi-adherent or suspension cells for imaging by immunofluorescence is challenging.. This protocol enables staining of floating cells with minimal morphological distortions, as we demonstrate with the semi-adherent multiple myeloma cell line RPMI 8226.. We describe steps to better preserve structural details by fixing, permeabilizing, and staining cells in solution, while minimizing the number of centrifugation steps and centrifugation g-force.

    Article Title: Protocol for cytoskeleton staining of the semi-adherent multiple myeloma cell line RPMI 8226 by immunofluorescence
    Article Snippet: .. We immunostained the RPMI 8226 cells with TUBB4A antibodies at a final concentration of 10 μg /mL from 1:30 dilution of a 300 μg /mL stock concentration obtained following the probe conjugation with Biotium CF568 Mix-n-Stain kit according to the manufacturer’s instructions: https://biotium.com/wp-content/uploads/2017/10/PI-Mix-n-Stain-Antibody-Labeling-Kits.pdf . c. Cover the Eppendorf tubes with aluminum foil to prevent light exposure and incubate them horizontally ∼15 h on a rocking platform (VWR Model 100 at the setting 2) at 4°C. ..

    Conjugation Assay:

    Article Title: Protocol for cytoskeleton staining of the semi-adherent multiple myeloma cell line RPMI 8226 by immunofluorescence.
    Article Snippet: Approach for minimal distortion of cell morphology by reducing centrifugation Preservation of fine cellular details of semi-adherent or suspension cells for imaging by immunofluorescence is challenging.. This protocol enables staining of floating cells with minimal morphological distortions, as we demonstrate with the semi-adherent multiple myeloma cell line RPMI 8226.. We describe steps to better preserve structural details by fixing, permeabilizing, and staining cells in solution, while minimizing the number of centrifugation steps and centrifugation g-force.

    Article Title: Protocol for cytoskeleton staining of the semi-adherent multiple myeloma cell line RPMI 8226 by immunofluorescence
    Article Snippet: .. We immunostained the RPMI 8226 cells with TUBB4A antibodies at a final concentration of 10 μg /mL from 1:30 dilution of a 300 μg /mL stock concentration obtained following the probe conjugation with Biotium CF568 Mix-n-Stain kit according to the manufacturer’s instructions: https://biotium.com/wp-content/uploads/2017/10/PI-Mix-n-Stain-Antibody-Labeling-Kits.pdf . c. Cover the Eppendorf tubes with aluminum foil to prevent light exposure and incubate them horizontally ∼15 h on a rocking platform (VWR Model 100 at the setting 2) at 4°C. ..



    Similar Products

    mix n stain cf568 antibody labeling kit  (Biotium)
    95
    Biotium mix n stain cf568 antibody labeling kit
    Mix N Stain Cf568 Antibody Labeling Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cf568+mix+n+stain+kit/Mix-n-Stain+Biotin+Antibody+Labeling+Kit/bio_rxiv__2025__09__12__675813-48-11-16
    Average 95 stars, based on 1 article reviews
    mix n stain cf568 antibody labeling kit - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    mix n stain cf568 dye antibody labeling kit  (Biotium)
    95
    Biotium mix n stain cf568 dye antibody labeling kit
    Mix N Stain Cf568 Dye Antibody Labeling Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cf568+mix+n+stain+kit/Mix-n-Stain+Biotin+Antibody+Labeling+Kit/pm40415024-514-15-20
    Average 95 stars, based on 1 article reviews
    mix n stain cf568 dye antibody labeling kit - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    mix n stain tm cf568 dye antibody labeling kit  (Biotium)
    93
    Biotium mix n stain tm cf568 dye antibody labeling kit
    Mix N Stain Tm Cf568 Dye Antibody Labeling Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cf568+mix+n+stain+kit/Mix-n-Stain+CF350+Antibody+Labeling+Kit/bio_rxiv__2024__10__09__617493-246-14-21
    Average 93 stars, based on 1 article reviews
    mix n stain tm cf568 dye antibody labeling kit - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    mix-n-stain cf568 antibody labeling kit  (Millipore)
    90
    Millipore mix-n-stain cf568 antibody labeling kit
    Mix N Stain Cf568 Antibody Labeling Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cf568+mix+n+stain+kit/donkey+anti+rabbit+immunoglobulin+g+antibody+conjugated+cf+568+fluorescence/pmc11322608-391-22-27
    Average 90 stars, based on 1 article reviews
    mix-n-stain cf568 antibody labeling kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    cf568 mix n stain kit  (Biotium)
    92
    Biotium cf568 mix n stain kit
    Immunofluorescence staining of RPMI 8226 trRpn13-MM2 cells Z-slices from a confocal microscopy image of a sample prepared following the described method showing RPMI 8226 trRpn13-MM2 cells undergoing mitosis (left) and at interphase (right) with fluorescence staining for F-actin (phalloidin-ATTO643, red), β-tubulin <t>(TUBB4A-CF568,</t> yellow) and DNA (DAPI, blue). Membrane blebbing is indicated by a white arrow. Scale bar is 2 μm.
    Cf568 Mix N Stain Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cf568+mix+n+stain+kit/https%3A%2F%2Fbiotium%2Ecom%2Fproduct%2Fsynaptoredtmc2m-also-known-as-fm5-95-a-trademark-of-molecular-probes-inc/pmc11078693-139-34-33
    Average 92 stars, based on 1 article reviews
    cf568 mix n stain kit - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier
    biotium cf568 mix n stain kit  (Biotium)
    92
    Biotium biotium cf568 mix n stain kit
    Immunofluorescence staining of RPMI 8226 trRpn13-MM2 cells Z-slices from a confocal microscopy image of a sample prepared following the described method showing RPMI 8226 trRpn13-MM2 cells undergoing mitosis (left) and at interphase (right) with fluorescence staining for F-actin (phalloidin-ATTO643, red), β-tubulin <t>(TUBB4A-CF568,</t> yellow) and DNA (DAPI, blue). Membrane blebbing is indicated by a white arrow. Scale bar is 2 μm.
    Biotium Cf568 Mix N Stain Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cf568+mix+n+stain+kit/https%3A%2F%2Fbiotium%2Ecom%2Fproduct%2Fsynaptoredtmc2m-also-known-as-fm5-95-a-trademark-of-molecular-probes-inc/pm38700979-90-33-33
    Average 92 stars, based on 1 article reviews
    biotium cf568 mix n stain kit - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Immunofluorescence staining of RPMI 8226 trRpn13-MM2 cells Z-slices from a confocal microscopy image of a sample prepared following the described method showing RPMI 8226 trRpn13-MM2 cells undergoing mitosis (left) and at interphase (right) with fluorescence staining for F-actin (phalloidin-ATTO643, red), β-tubulin (TUBB4A-CF568, yellow) and DNA (DAPI, blue). Membrane blebbing is indicated by a white arrow. Scale bar is 2 μm.

    Journal: STAR Protocols

    Article Title: Protocol for cytoskeleton staining of the semi-adherent multiple myeloma cell line RPMI 8226 by immunofluorescence

    doi: 10.1016/j.xpro.2024.103060

    Figure Lengend Snippet: Immunofluorescence staining of RPMI 8226 trRpn13-MM2 cells Z-slices from a confocal microscopy image of a sample prepared following the described method showing RPMI 8226 trRpn13-MM2 cells undergoing mitosis (left) and at interphase (right) with fluorescence staining for F-actin (phalloidin-ATTO643, red), β-tubulin (TUBB4A-CF568, yellow) and DNA (DAPI, blue). Membrane blebbing is indicated by a white arrow. Scale bar is 2 μm.

    Article Snippet: We immunostained the RPMI 8226 cells with TUBB4A antibodies at a final concentration of 10 μg /mL from 1:30 dilution of a 300 μg /mL stock concentration obtained following the probe conjugation with Biotium CF568 Mix-n-Stain kit according to the manufacturer’s instructions: https://biotium.com/wp-content/uploads/2017/10/PI-Mix-n-Stain-Antibody-Labeling-Kits.pdf . c. Cover the Eppendorf tubes with aluminum foil to prevent light exposure and incubate them horizontally ∼15 h on a rocking platform (VWR Model 100 at the setting 2) at 4°C.

    Techniques: Immunofluorescence, Staining, Confocal Microscopy, Fluorescence, Membrane